Details

Autor(en) / Beteiligte

• Kubicz, Ewelina
• Jasińska, Bożena
• Zgardzińska, Bożena
• Bednarski, Tomasz
• Białas, Piotr
• Czerwiński, Eryk
• Gajos, Aleksander
• Gorgol, Marek
• Kamińska, Daria
• Kapłon, Łukasz
• Kochanowski, Andrzej
• Korcyl, Grzegorz
• Kowalski, Paweł
• Kozik, Tomasz
• Krzemień, Wojciech
• Niedźwiecki, Szymon
• Pałka, Marek
• Raczyński, Lech
• Rajfur, Zenon
• Rudy, Zbigniew
• Rundel, Oleksandr
• Sharma, Neha Gupta
• Silarski, Michał
• Słomski, Artur
• Strzelecki, Adam
• Wieczorek, Anna
• Wiślicki, Wojciech
• Zieliński, Marcin
• Moskal, Paweł

Titel

Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy

Ist Teil von

• Nukleonika, 2015-12, Vol.60 (4), p.749-753

Ort / Verlag

De Gruyter Open

Erscheinungsjahr

2015

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of -positronium ( -Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of -Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images.