Nature communications, 2019-10, Vol.10 (1), p.4706-16, Article 4706
2019
Details
- Autor(en) / Beteiligte
- Titel
- Single-cell transcriptomics of human T cells reveals tissue and activation signatures in health and disease
- Ist Teil von
- Nature communications, 2019-10, Vol.10 (1), p.4706-16, Article 4706
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Human T cells coordinate adaptive immunity in diverse anatomic compartments through production of cytokines and effector molecules, but it is unclear how tissue site influences T cell persistence and function. Here, we use single cell RNA-sequencing (scRNA-seq) to define the heterogeneity of human T cells isolated from lungs, lymph nodes, bone marrow and blood, and their functional responses following stimulation. Through analysis of >50,000 resting and activated T cells, we reveal tissue T cell signatures in mucosal and lymphoid sites, and lineage-specific activation states across all sites including distinct effector states for CD8 T cells and an interferon-response state for CD4 T cells. Comparing scRNA-seq profiles of tumor-associated T cells to our dataset reveals predominant activated CD8 compared to CD4 T cell states within multiple tumor types. Our results therefore establish a high dimensional reference map of human T cell activation in health for analyzing T cells in disease.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-019-12464-3Titel-ID: cdi_doaj_primary_oai_doaj_org_article_630f23689fa444fe9d811c61653d4606
- Format
- –
- Schlagworte
- Adaptive immunity, Bone marrow, CD4 antigen, CD4-Positive T-Lymphocytes - immunology, CD4-Positive T-Lymphocytes - metabolism, CD8 antigen, CD8-Positive T-Lymphocytes - immunology, CD8-Positive T-Lymphocytes - metabolism, Cell activation, Cells, Cultured, Cytokines, Gene sequencing, Heterogeneity, Humans, Interferon, Lung - metabolism, Lymph nodes, Lymph Nodes - immunology, Lymph Nodes - metabolism, Lymphocyte Activation - genetics, Lymphocyte Activation - immunology, Lymphocytes, Lymphocytes T, Mucosa, Mucous Membrane - immunology, Mucous Membrane - metabolism, Neoplasms - genetics, Neoplasms - pathology, Ribonucleic acid, RNA, Signatures, Single-Cell Analysis - methods, T-Lymphocytes - immunology, T-Lymphocytes - metabolism, Tissues, Transcriptome - genetics, Tumors