Nature communications, 2021-11, Vol.12 (1), p.6639-6639, Article 6639
2021
Details
- Autor(en) / Beteiligte
- Titel
- Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry
- Ist Teil von
- Nature communications, 2021-11, Vol.12 (1), p.6639-6639, Article 6639
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view of ~25 mm , encompassing distances up to 7 mm, with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-021-26736-4Titel-ID: cdi_doaj_primary_oai_doaj_org_article_61e2b203f08348f7bc349521ead6e6c7
- Format
- –
- Schlagworte
- Adaptive optics, Brain, Brain - cytology, Brain - physiology, Calcium, Calcium - metabolism, Calcium imaging, Circuits, Engines, Equipment Design, Field of view, Imaging, Microscopy, Fluorescence, Multiphoton - instrumentation, Microscopy, Fluorescence, Multiphoton - methods, Multiplexing, Nerve Net - cytology, Nerve Net - physiology, Neural networks, Neuroimaging, Neurons, Neurons - cytology, Neurons - physiology, Optical design, Optical Imaging - instrumentation, Optical Imaging - methods, Optics, Photons