Details

Autor(en) / Beteiligte

• Patel, Akshar
• Simkulet, Matthew
• Maity, Soumya
• Venkatesan, Manigandan
• Matzavinos, Anastasios
• Madesh, Muniswamy
• Alevriadou, B. Rita

Titel

The mitochondrial Ca2+ uniporter channel synergizes with fluid shear stress to induce mitochondrial Ca2+ oscillations

Ist Teil von

• Scientific reports, 2022-12, Vol.12 (1), p.21161-21161, Article 21161

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

2022

Quelle

EZB*

Beschreibungen/Notizen

• Abstract The mitochondrial calcium (Ca 2+ ) uniporter (MCU) channel is responsible for mitochondrial Ca 2+ influx. Its expression was found to be upregulated in endothelial cells (ECs) under cardiovascular disease conditions. Since the role of MCU in regulating cytosolic Ca 2+ homeostasis in ECs exposed to shear stress (SS) is unknown, we studied mitochondrial Ca 2+ dynamics (that is known to decode cytosolic Ca 2+ signaling) in sheared ECs. To understand cause-and-effect, we ectopically expressed MCU in ECs. A higher percentage of MCU-transduced ECs exhibited mitochondrial Ca 2+ transients/oscillations, and at higher frequency, under SS compared to sheared control ECs. Transients/oscillations correlated with mitochondrial reactive oxygen species (mROS) flashes and mitochondrial membrane potential (ΔΨ m ) flickers, and depended on activation of the mechanosensitive Piezo1 channel and the endothelial nitric oxide synthase (eNOS). A positive feedback loop composed of mitochondrial Ca 2+ uptake/mROS flashes/ΔΨ m flickers and endoplasmic reticulum Ca 2+ release, in association with Piezo1 and eNOS, provided insights into the mechanism by which SS, under conditions of high MCU activity, may shape vascular EC energetics and function.