Details

Autor(en) / Beteiligte

• Forsdick, Natalie J.
• Martini, Denise
• Brown, Liz
• Cross, Hugh B.
• Maloney, Richard F.
• Steeves, Tammy E.
• Knapp, Michael

Titel

Genomic sequencing confirms absence of introgression despite past hybridisation between a critically endangered bird and its common congener

Ist Teil von

• Global ecology and conservation, 2021-08, Vol.28, p.e01681, Article e01681

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Genetic swamping resulting from interspecific hybridisation can increase extinction risk for threatened species. The development of high-throughput and reduced-representation genomic sequencing and analyses to generate large numbers of high resolution genomic markers has the potential to reveal introgression previously undetected using small numbers of genetic markers. However, few studies to date have implemented genomic tools to assess the extent of interspecific hybridisation in threatened species. Here we investigate the utility of genome-wide single nucleotide polymorphisms (SNPs) to detect introgression resulting from past interspecific hybridisation in one of the world’s rarest birds. Anthropogenic impacts have resulted in hybridisation and subsequent backcrossing of the critically endangered Aotearoa New Zealand endemic kakī (black stilts; Himantopus novaezelandiae) with the non-threatened self-introduced congeneric poaka (Aotearoa New Zealand population of pied stilts, Himantopus himantopus leucocephalus), yet genetic analyses with a limited set of microsatellite markers revealed no evidence of introgression of poaka genetic material in kakī, excluding one individual. We use genomic data for ~63% of the wild adult kakī population to reassess the extent of introgression resulting from hybridisation between kakī and poaka. Consistent with previous genetic analyses, we detected no introgression from poaka into kakī. These collective results indicate that, for kakī, existing microsatellite markers provide a robust, cost-effective approach to detect cryptic hybrids. Further, for well-differentiated species, the use of genomic markers may not be required to detect admixed individuals. [Display omitted] •Few genomic studies have explored the impact of hybridisation on threatened species.•Critically endangered kakī have been negatively impacted by hybridisation with poaka.•Previous genetic data found limited introgression into kakī.•Using genomic SNPs we find no introgression into kakī, consistent with genetic data.•Genetic data remain a cost-effective tool to identify cryptic hybrids for conservation management.