Scientific reports, 2017-12, Vol.7 (1), p.17071-20, Article 17071
2017
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- Autor(en) / Beteiligte
- Titel
- Deep tissue analysis of distal aqueous drainage structures and contractile features
- Ist Teil von
- Scientific reports, 2017-12, Vol.7 (1), p.17071-20, Article 17071
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2017
- Quelle
- EZB-FREE-00999 freely available EZB journals
- Beschreibungen/Notizen
- Outflow resistance in the aqueous drainage tract distal to trabecular meshwork is potentially an important determinant of intraocular pressure and success of trabecular bypass glaucoma surgeries. It is unclear how distal resistance is modulated. We sought to establish: (a) multimodal 2-photon deep tissue imaging and 3-dimensional analysis of the distal aqueous drainage tract (DT) in transgenic mice in vivo and ex vivo; (b) criteria for distinguishing the DT from blood and lymphatic vessels; and (c) presence of a DT wall organization capable of contractility. DT lumen appeared as scleral collagen second harmonic generation signal voids that could be traced back to Schlemm's canal. DT endothelium was Prox1-positive, CD31-positive and LYVE-1-negative, bearing a different molecular signature from blood and true lymphatic vessels. DT walls showed prominent filamentous actin (F-actin) labeling reflecting cells in a contracted state. F-actin co-localized with mesenchymal smooth muscle epitopes of alpha-smooth muscle actin, caldesmon and calponin, which localized adjacent and external to the endothelium. Our findings support a DT wall organization resembling that of blood vessels. This reflects a capacity to contract and support dynamic alteration of DT caliber and resistance analogous to the role of blood vessel tone in regulating blood flow.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/s41598-017-16897-yTitel-ID: cdi_doaj_primary_oai_doaj_org_article_5e9d8604e15246c3a3f8ff47cabada08
- Format
- –
- Schlagworte
- Actin, Actins - metabolism, Animals, Aqueous Humor - metabolism, Blood flow, Blood vessels, Calcium-Binding Proteins - metabolism, Calmodulin-Binding Proteins - metabolism, Calponin, Calponins, Collagen, Dimensional analysis, Endothelium, Endothelium - metabolism, Epitopes, Glaucoma, Homeodomain Proteins - metabolism, Lymphatic system, Lymphatic Vessels - pathology, Mesenchyme, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Microfilament Proteins - metabolism, Microscopy, Fluorescence, Multiphoton, Muscle Contraction, Muscle, Smooth - pathology, Muscle, Smooth - physiology, Rodents, Sclera - metabolism, Sclera - ultrastructure, Smooth muscle, Trabecular Meshwork - metabolism, Trabecular Meshwork - ultrastructure, Transgenic mice, Tumor Suppressor Proteins - metabolism, Wound drainage