Details

Autor(en) / Beteiligte

• Lenz, Swantje
• Sinn, Ludwig R.
• O’Reilly, Francis J.
• Fischer, Lutz
• Wegner, Fritz
• Rappsilber, Juri

Titel

Reliable identification of protein-protein interactions by crosslinking mass spectrometry

Ist Teil von

• Nature communications, 2021-06, Vol.12 (1), p.3564-3564, Article 3564

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Abstract Protein-protein interactions govern most cellular pathways and processes, and multiple technologies have emerged to systematically map them. Assessing the error of interaction networks has been a challenge. Crosslinking mass spectrometry is currently widening its scope from structural analyses of purified multi-protein complexes towards systems-wide analyses of protein-protein interactions (PPIs). Using a carefully controlled large-scale analysis of Escherichia coli cell lysate, we demonstrate that false-discovery rates (FDR) for PPIs identified by crosslinking mass spectrometry can be reliably estimated. We present an interaction network comprising 590 PPIs at 1% decoy-based PPI-FDR. The structural information included in this network localises the binding site of the hitherto uncharacterised protein YacL to near the DNA exit tunnel on the RNA polymerase.