International journal of molecular sciences, 2009-04, Vol.10 (4), p.1930-1941
2009
Details
- Autor(en) / Beteiligte
- Titel
- Fluorescence lifetime imaging of quantum dot labeled DNA microarrays
- Ist Teil von
- International journal of molecular sciences, 2009-04, Vol.10 (4), p.1930-1941
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2009
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms10041930Titel-ID: cdi_doaj_primary_oai_doaj_org_article_5d5e4d5e2839426f84fd10805e08aed3
- Format
- –
- Schlagworte
- Cytomegalovirus - genetics, DNA - chemistry, DNA - metabolism, DNA Microarray, DNA, Viral - chemistry, DNA, Viral - metabolism, Dyes, Electronic mail systems, Fluorescence Lifetime Imaging Microscopy (FLIM), Hepacivirus - genetics, Hepatitis C, Humans, Labeling, Lifetime Multiplexing, Microscopy, Microscopy, Fluorescence, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis, Quantum Dots, Quantum Dots (QD), RNA, Viral - chemistry, RNA, Viral - metabolism