Details

Autor(en) / Beteiligte

• Elsaid, Ramy
• Mikdache, Aya
• Castillo, Keinis Quintero
• Salloum, Yazan
• Diabangouaya, Patricia
• Gros, Gwendoline
• Feijoo, Carmen G.
• Hernández, Pedro P.

Titel

Definitive hematopoiesis is dispensable to sustain erythrocytes and macrophages during zebrafish ontogeny

Ist Teil von

• iScience, 2024-02, Vol.27 (2), p.108922-108922, Article 108922

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• In all organisms studied, from flies to humans, blood cells emerge in several sequential waves and from distinct hematopoietic origins. However, the relative contribution of these ontogenetically distinct hematopoietic waves to embryonic blood lineages and to tissue regeneration during development is yet elusive. Here, using a lineage-specific “switch and trace” strategy in the zebrafish embryo, we report that the definitive hematopoietic progeny barely contributes to erythrocytes and macrophages during early development. Lineage tracing further shows that ontogenetically distinct macrophages exhibit differential recruitment to the site of injury based on the developmental stage of the organism. We further demonstrate that primitive macrophages can solely maintain tissue regeneration during early larval developmental stages after selective ablation of definitive macrophages. Our findings highlight that the sequential emergence of hematopoietic waves in embryos ensures the abundance of blood cells required for tissue homeostasis and integrity during development. [Display omitted] •Primitive hematopoiesis gives rise to and sustains embryonic erythromyeloid lineages•Primitive macrophages are recruited earlier to the site of injury•Ablation of larval definitive macrophages does not impair tail fin regeneration•Primitive macrophages depletion impairs tail fin regeneration in zebrafish larvae Biological sciences; Developmental biology; Embryology