Genome Biology, 2020-10, Vol.21 (1), p.257-257, Article 257
2020
Details
- Autor(en) / Beteiligte
- Titel
- Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
- Ist Teil von
- Genome Biology, 2020-10, Vol.21 (1), p.257-257, Article 257
- Ort / Verlag
- England: BioMed Central
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1474-760X, 1474-7596eISSN: 1474-760XDOI: 10.1186/s13059-020-02170-5Titel-ID: cdi_doaj_primary_oai_doaj_org_article_5ae751de190c46b0a5d55075e84a8549
- Format
- –
- Schlagworte
- Acetolactate Synthase - genetics, ALS, Binding sites, Cloning, CRISPR, CRISPR/Cas9, Deoxyribonucleic acid, DNA, DNA repair, Efficiency, Gene Editing - methods, Gene Editing - statistics & numerical data, Genetic Vectors, Genomes, Harbors, Maize, Mutagenesis, Site-Directed - methods, Mutation, PE2, pegRNA, Plants, Genetically Modified, Prime editing, RNA, Guide, CRISPR-Cas Systems - genetics, RNA, Guide, CRISPR-Cas Systems - metabolism, Short Report, Zea mays - enzymology, Zea mays - genetics