Details

Autor(en) / Beteiligte

• Mistegård, Clara Elbæk
• Troldborg, Anne
• Loft, Anne Gitte
• Thiel, Steffen
• Spiller, Laura
• Protopopov, Mikhail
• Rios Rodriguez, Valeria
• Muche, Burkhard
• Rademacher, Judith
• Weber, Anne-Katrin
• Lüders, Susanne
• Sieper, Joachim
• Poddubnyy, Denis
• Proft, Fabian

Titel

Exploring complement biomarkers in suspected axial spondyloarthritis

Ist Teil von

• Rheumatic & musculoskeletal diseases open, 2024-05, Vol.10 (2), p.e004127

Ort / Verlag

England: EULAR

Erscheinungsjahr

2024

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• ObjectivesTo investigate lectin pathway proteins (LPPs) as biomarkers for axial spondyloarthritis (axSpA) in a cross-sectional cohort with a suspicion of axSpA, comprising newly diagnosed axSpA and chronic low back pain (cLBP) individuals.MethodsSerum samples from 515 participants within the OptiRef cohort, including 151 axSpA patients and 364 cLBP patients, were measured using immunoassays for LPPs (mannan-binding lectin (MBL), collectin liver-1 (CL-L1), M-ficolin, H-ficolin and L-ficolin, MBL-associated serine proteases (MASP)−1, –2 and –3, MBL-associated proteins (MAp19 and MAp44) and the complement activation product C3dg).ResultsSerum levels of L-ficolin, MASP-2 and C3dg were elevated in axSpA patients, whereas levels of MASP-3 and CL-L1 were decreased, and this remained significant for C3dg and MASP-3 after adjustment for C reactive protein (CRP). A univariate regression analysis showed serum levels of CL-L1, MASP-2, MASP-3 and C3dg to predict the diagnosis of axSpA, and MASP-3 and C3dg remained significant in a multivariate logistic regression analysis. Assessment of the diagnostic potential showed that a combination of human leukocyte antigen B27 (HLA-B27) and measurements of L-ficolin, MASP-3 and C3dg increased the diagnostic specificity for axSpA, however, with a concomitant loss of sensitivity.ConclusionsSerum levels of complement activation, that is, C3dg, and MASP-3 differed significantly between axSpA and cLBP patients after adjustment for CRP. Although combining HLA-B27 with measurements of L-ficolin, MASP-3 and C3dg increased the diagnostic specificity for axSpA, this seems unjustified due to the concomitant loss of sensitivity. However, both C3dg and MASP-3 were associated with axSpA diagnosis in multivariate logistic regression, suggesting an involvement of complement in the inflammatory processes and possibly pathogenesis in axSpA.