Details

Autor(en) / Beteiligte

• Yang, Yufan
• Sun, Ying
• Wang, Zhaoxin
• Yin, Maojing
• Sun, Runze
• Xue, Lu
• Huang, Xueshuang
• Wang, Chunhua
• Yan, Xiaohui

Titel

Full-length transcriptome and metabolite analysis reveal reticuline epimerase-independent pathways for benzylisoquinoline alkaloids biosynthesis in Sinomenium acutum

Ist Teil von

• Frontiers in plant science, 2022-12, Vol.13, p.1086335-1086335

Ort / Verlag

Switzerland: Frontiers Media S.A

Erscheinungsjahr

2022

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Benzylisoquinoline alkaloids (BIAs) are a large family of plant natural products with important pharmaceutical applications. is a medicinal plant from the Menispermaceae family and has been used to treat rheumatoid arthritis for hundreds of years. contains more than 50 BIAs, and sinomenine is a representative BIA from this plant. Sinomenine was found to have preventive and curative effects on opioid dependence. Despite the broad applications of , investigation on the biosynthetic pathways of BIAs from is limited. In this study, we comprehensively analyzed the transcriptome data and BIAs in the root, stem, leaf, and seed of . Metabolic analysis showed a noticeable difference in BIA contents in different tissues. Based on the study of the full-length transcriptome, differentially expressed genes, and weighted gene co-expression network, we proposed the biosynthetic pathways for a few BIAs from , such as sinomenine, magnoflorine, and tetrahydropalmatine, and screened candidate genes involved in these biosynthesis processes. Notably, the reticuline epimerase (REPI/STORR), which converts ( )-reticuline to ( )-reticuline and plays an essential role in morphine and codeine biosynthesis, was not found in the transcriptome data of . Our results shed light on the biogenesis of the BIAs in and may pave the way for the future development of this important medicinal plant.