Details

Autor(en) / Beteiligte

• Asmamaw Mengstie, Misganaw
• Teshome Azezew, Muluken
• Asmamaw Dejenie, Tadesse
• Teshome, Assefa Agegnehu
• Tadele Admasu, Fitalew
• Behaile Teklemariam, Awgichew
• Tilahun Mulu, Anemut
• Mekonnen Agidew, Melaku
• Adugna, Dagnew Getnet
• Geremew, Habtamu
• Abebe, Endeshaw Chekol

Titel

Recent Advancements in Reducing the Off-Target Effect of CRISPR-Cas9 Genome Editing

Ist Teil von

• Biologics, 2024-01, Vol.18, p.21-28

Ort / Verlag

New Zealand: Dove Medical Press Limited

Erscheinungsjahr

2024

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)) and the associated protein (Cas9) system, a young but well-studied genome-editing tool, holds plausible solutions to a wide range of genetic disorders. The single-guide RNA (sgRNA) with a 20-base user-defined spacer sequence and the Cas9 endonuclease form the core of the CRISPR-Cas9 system. This sgRNA can direct the Cas9 nuclease to any genomic region that includes a protospacer adjacent motif (PAM) just downstream and matches the spacer sequence. The current challenge in the clinical applications of CRISPR-Cas9 genome-editing technology is the potential off-target effects that can cause DNA cleavage at the incorrect sites. Off-target genome editing confuses and diminishes the therapeutic potential of CRISPR-Cas9 in addition to potentially casting doubt on scientific findings regarding the activities of genes. In this review, we summarize the recent technological advancements in reducing the off-target effect of CRISPR-Cas9 genome editing.