Details

Autor(en) / Beteiligte

• Zhang, Chun-ge
• Zhu, Qiao-ling
• Zhou, Yi
• Liu, Yang
• Chen, Wei-liang
• Yuan, Zhi-Qiang
• Yang, Shu-di
• Zhou, Xiao-feng
• Zhu, Ai-jun
• Zhang, Xue-nong
• Jin, Yong

Titel

N-Succinyl-chitosan nanoparticles coupled with low-density lipoprotein for targeted osthole-loaded delivery to low-density lipoprotein receptor-rich tumors

Ist Teil von

• International journal of nanomedicine, 2014-01, Vol.9 (Issue 1), p.2919-2932

Ort / Verlag

New Zealand: Dove Medical Press Limited

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• N-Succinyl-chitosan (NSC) was synthesized and NSC nanoparticles (NPs) with loaded osthole (Ost) (Ost/NSC-NPs) were prepared by emulsion solvent diffusion. Subsequently, low-density lipoprotein (LDL)-mediated NSC-NPs with loaded Ost (Ost/LDL-NSC-NPs) were obtained by coupling LDL with Ost/NSC-NPs through amide linkage. The average particle size of Ost/NSC-NPs was approximately 145 nm, the entrapment efficiency was 78.28%±2.06%, and the drug-loading amount was 18.09%±0.17%. The release of Ost from Ost/NSC-NPs in vitro showed a more evident sustained effect than the native material. The half maximal inhibitory concentration of Ost/LDL-NSC-NPs was only 16.23% that of the free Ost at 24 hours in HepG2 cells. Ost inhibited HepG2 cell proliferation by arresting cells in the synthesis phase of the cell cycle and by triggering apoptosis. Cellular uptake and subcellular localization in vitro and near-infrared fluorescence real-time imaging in vivo showed that Ost/LDL-NSC-NPs had high targeting efficacy. Therefore, LDL-NSC-NPs are a promising system for targeted Ost delivery to liver tumor.