Details
- Autor(en) / Beteiligte
- Titel
- A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling
- Ist Teil von
- eLife, 2020-12, Vol.9
- Ort / Verlag
- England: eLife Science Publications, Ltd
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Phosphoinositides (PI) are key regulators of cellular organization in eukaryotes and genes that tune PI signaling are implicated in human disease mechanisms. Biochemical analyses and studies in cultured cells have identified a large number of proteins that can mediate PI signaling. However, the role of such proteins in regulating cellular processes and development in metazoans remains to be understood. Here, we describe a set of CRISPR-based genome engineering tools that allow the manipulation of each of these proteins with spatial and temporal control during metazoan development. We demonstrate the use of these reagents to deplete a set of 103 proteins individually in the eye and identify several new molecules that control eye development. Our work demonstrates the power of this resource in uncovering the molecular basis of tissue homeostasis during normal development and in human disease biology.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/ELIFE.55793Titel-ID: cdi_doaj_primary_oai_doaj_org_article_55d03f35196145a298b499fab390966c
- Format
- –
- Schlagworte
- Analysis, Animals, Clustered Regularly Interspaced Short Palindromic Repeats - genetics, CRISPR-Cas Systems - genetics, CRISPR/Cas9, Developmental Biology, Drosophila, Drosophila melanogaster - embryology, Drosophila melanogaster - genetics, Eye - embryology, Eye - metabolism, eye development, Gene Editing - methods, Gene Knockout Techniques, Genes, Genetic Engineering - methods, Genetics and Genomics, genome engineering, Genome, Insect - genetics, Genomes, Genomics, Lipid Metabolism, Medical research, Medicine, Experimental, Phosphatidylinositols - metabolism, Proteins, RNA, Guide, CRISPR-Cas Systems - biosynthesis, RNA, Guide, CRISPR-Cas Systems - genetics, Sequence Deletion - genetics, Signal Transduction - physiology, Tools and Resources