Details
- Autor(en) / Beteiligte
- Titel
- Simultaneous two-photon imaging and two-photon optogenetics of cortical circuits in three dimensions
- Ist Teil von
- eLife, 2018-02, Vol.7
- Ort / Verlag
- England: eLife Sciences Publications Ltd
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- The simultaneous imaging and manipulating of neural activity could enable the functional dissection of neural circuits. Here we have combined two-photon optogenetics with simultaneous volumetric two-photon calcium imaging to measure and manipulate neural activity in mouse neocortex in vivo in three-dimensions (3D) with cellular resolution. Using a hybrid holographic approach, we simultaneously photostimulate more than 80 neurons over 150 μm in depth in layer 2/3 of the mouse visual cortex, while simultaneously imaging the activity of the surrounding neurons. We validate the usefulness of the method by photoactivating in 3D selected groups of interneurons, suppressing the response of nearby pyramidal neurons to visual stimuli in awake animals. Our all-optical approach could be used as a general platform to read and write neuronal activity.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/elife.32671Titel-ID: cdi_doaj_primary_oai_doaj_org_article_5405d31bc61c4404af680d0c7a243d25
- Format
- –
- Schlagworte
- Animals, Calcium imaging, Circuits, Functional morphology, Genetics, holographic, Imaging, Three-Dimensional, Information processing, Interneurons, Lasers, Mice, Microscopy, Microscopy, Fluorescence, Multiphoton, Mirrors, Neural networks, Neural Pathways - anatomy & histology, Neural Pathways - physiology, Neuroimaging, Neuroscience, Optics, Optogenetics, Pyramidal cells, three-dimensional, Tools and Resources, two-photon, Visual cortex, Visual Cortex - anatomy & histology, Visual Cortex - physiology, Visual pathways, Visual stimuli, volumetric