Details

Autor(en) / Beteiligte

• Yamashita, Yoshiro
• Yasuda, Ikkoh
• Tanaka, Takeshi
• Ikeda, Toru
• Terada, Mayumi
• Takaki, Masahiro
• Tsuchihashi, Yoshiko
• Asoh, Norichika
• Ohara, Yukiko
• Enany, Shymaa
• Kobayashi, Haruka
• Matsumoto, Sohkichi
• Morimoto, Konosuke

Titel

Antigen-specific cytokine profiles for pulmonary Mycobacterium avium complex disease stage diagnosis

Ist Teil von

• Frontiers in immunology, 2023-07, Vol.14, p.1222428-1222428

Ort / Verlag

Switzerland: Frontiers Media S.A

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Controlling pulmonary complex (MAC) disease is difficult because there is no way to know the clinical stage accurately. There have been few attempts to use cell-mediated immunity for diagnosing the stage. The objective of this study was to characterize cytokine profiles of CD4+T and CD19+B cells that recognize various -associated antigens in different clinical stages of MAC. A total of 47 MAC patients at different stages based on clinical information (14 before-treatment, 16 on-treatment, and 17 after-treatment) and 17 healthy controls were recruited. Peripheral blood mononuclear cells were cultured with specific antigens (MAV0968, 1160, 1276, and 4925), and the cytokine profiles (IFN-γ, TNF-α, IL-2, IL-10, IL-13, and IL-17) of CD4+/CD3+ and CD19+ cells were analyzed by flow cytometry. The response of Th1 cytokines such as IFN-γ and TNF-α against various antigens was significantly higher in both the on-treatment and after-treatment groups than in the before-treatment group and control (P < 0.01-0.0001 and P < 0.05-0.0001). An analysis of polyfunctional T cells suggested that the presence of IL-2 is closely related to the stage after the start of treatment (P = 0.0309-P < 0.0001) and is involved in memory function. Non-Th1 cytokines, such as IL-10 and IL-17, showed significantly higher responses in the before-treatment group (P < 0.0001 and P < 0.01-0.0001). These responses were not observed with purified protein derivative (PPD). CD19+B cells showed a response similar to that of CD4+T cells. There is a characteristic cytokine profile at each clinical stage of MAC.