Molecular plant-microbe interactions, 2010-03, Vol.23 (3), p.294-303
2010
Details
- Autor(en) / Beteiligte
- Titel
- Cucumber Mosaic Virus 2b Protein Subcellular Targets and Interactions: Their Significance to RNA Silencing Suppressor Activity
- Ist Teil von
- Molecular plant-microbe interactions, 2010-03, Vol.23 (3), p.294-303
- Ort / Verlag
- St. Paul, MN: APS Press
- Erscheinungsjahr
- 2010
- Link zum Volltext
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- The RNA silencing suppressor activity of the 2b protein of Cucumber mosaic virus (CMV) has been variously attributed to its nuclear targeting, its interaction with and inhibition of Argonaute 1 (AGO1), or its ability to bind small RNAs in vitro. In addition, the 2b ortholog of Tomato aspermy virus forms aggregates and binds RNAs in vitro. We have further studied the relationships between CMV 2b protein silencing suppressor activity and its subcellular distribution, protein-protein interactions in vivo, and interactions with small interfering RNAs in vitro. To do this, we tagged the protein with fluorescent markers and showed that it retained suppressor activity. We showed that the 2b protein is present in the nucleolus and that it self-interacts and interacts with AGO1 and AGO4 in vivo. Using a battery of mutants, we showed that the putative nuclear localization signals and phosphorylation motif of the 2b protein are not required for self-interaction or for interaction with AGO proteins. The occurrence of neither of these interactions or of nucleolar targeting was sufficient to provide local silencing-suppression activity. In contrast, the ability of the 2b protein to bind small RNAs appears to be indispensable for silencing suppressor function.
- Sprache
- Englisch; Russisch
- Identifikatoren
- ISSN: 0894-0282eISSN: 1943-7706DOI: 10.1094/MPMI-23-3-0294Titel-ID: cdi_doaj_primary_oai_doaj_org_article_532e9f1294f3405db6fb2d2683786c21
- Format
- –
- Schlagworte
- active transport, cell nucleolus, Cucumber mosaic virus, Cucumovirus - genetics, Cucumovirus - metabolism, cytochemistry, Cytomegalovirus, defense mechanisms, Fluorescent indicators, gene expression regulation, gene silencing, in vitro studies, in vivo studies, Luminescent Proteins - genetics, Luminescent Proteins - metabolism, Lycopersicon esculentum, microbial genetics, mutants, Mutation, Nicotiana - genetics, Nicotiana - metabolism, Nicotiana - virology, Nuclear Localization Signals - genetics, Nucleoli, Phosphorylation, Plant Leaves - genetics, Plant Leaves - metabolism, Plant Leaves - virology, plant proteins, Plant Proteins - genetics, Plant Proteins - metabolism, Protein Binding, Protein interaction, protein phosphorylation, protein-protein interactions, resistance mechanisms, RNA Interference, RNA silencing suppressor activity, RNA, Small Interfering - genetics, RNA, Small Interfering - metabolism, RNA-mediated interference, siRNA, small interfering RNA, viral proteins, Viral Proteins - genetics, Viral Proteins - metabolism