Details

Autor(en) / Beteiligte

• Zhang, Jinna
• Chen, Rucheng
• Zhang, Guoqing
• Wang, Yixuan
• Peng, Jing
• Hu, Renjie
• Li, Ran
• Gu, Weijia
• Zhang, Lu
• Sun, Qinghua
• Liu, Cuiqing

Titel

PM2.5 increases mouse blood pressure by activating toll-like receptor 3

Ist Teil von

• Ecotoxicology and environmental safety, 2022-04, Vol.234, p.113368, Article 113368

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2022

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Plenty of literature has documented that fine particulate matter (PM2.5) exposure is related to blood pressure (BP) elevation. Vascular dysfunction is the initiation of cardiovascular diseases, such as hypertension. This thesis set out to assess the role of Toll-like receptor 3 (TLR3) in the increase in BP induced by PM2.5. C57BL/6 and TLR3 deficient (TLR3-/-) male mice were randomly allocated to filtered air chamber or real-world inhaled concentrated PM2.5 chamber. BP was evaluated using non-invasive BP recordings. After euthanasia, the aortas and small mesenteric arteries (SMAs) were isolated, and vascular tone was measured using a wire myograph. Leucocytes were detached to assess myeloid-derived suppressor cells using flow cytometry. siRNA transfection was performed to silence TLR3 expression in the human vascular endothelial cells incubated with PM2.5. The gene expression levels of inflammation, adhesion molecules, and oxidative stress in the aortas were assessed by quantitative PCR. Exposure to PM2.5 increased mouse BP, and TLR3 deficiency protected against PM2.5 exposure-induced BP increase. Additionally, the injury of vascular function in the aortas and SMAs was inhibited in TLR3-/- mice. The intercellular adhesion molecule-1 (ICAM-1) was attenuated in TLR3-/- mice, accompanied by the inhibition of inflammatory and oxidized genes of the aortas, such as F4/80, interleukin-6, interleukin-1 beta, and NADPH oxidase 4. In vitro, the enhanced mRNA expression of genes encoding inflammation, oxidative stress, and ICAM-1 by PM2.5 was inhibited by TLR3 silence as well. PM2.5 exposure increased BP via TLR3 activation and impaired vascular function. •PM2.5-elevated BP was attenuated by TLR3 deficiency.•PM2.5-impaired vascular function was improved by TLR3 deficiency.•PM2.5-induced inflammation and oxidative stress was inhibited by TLR3 deficiency.