Details

Autor(en) / Beteiligte

• Vorobey, Nadiya
• Kukol, Kateryna
• Pukhtaievych, Petro
• Kots, Sergii

Titel

Influence of carmoisine on the viability of Bradyrhizobium japonicum in vitro and physiological indices of soybean under symbiosis conditions

Ist Teil von

• Journal of Central European Agriculture, 2021-12, Vol.22 (4), p.735-747

Ort / Verlag

Sveuciliste U Zagrebu

Erscheinungsjahr

2021

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• The effect of carmoisine (azorubin) on the viability and reproduction of Bradyrhizobium japonicum (Kirchner, 1896), Jordan, 1982 in vitro and on the physiological parameters of soybean plants under conditions of symbiosis with the bacteria was studied. Sensitivity to carmoisine of selected strains of B. japonicum--634b, 646, 614, M8, PC07, PC09, PC10 and Tn5-mutants of B. japonicum 646--B20, B78, B144 was studied by the "hole method". It was determined that B. japonicum strains are not sensitive to carmoisine in concentrations of 0.25-1.0%, because when growing rhizobia on the surface of mannitol-yeast agar, the zones of inhibition of culture growth around the "holes" with solutions of synthetic colorant were absent. In pot experiments, the physiological response of Glycine max (L.) Merr. plants of the Almaz variety to inoculation was studied by biological preparations based on B. japonicum B78 and PC07 with carmoisine in their composition. It was shown that the studied biological products had no negative impact on plant growth and development compared to the control, and on the contrary, it contributed to the activization of biosynthetic processes, e.g., the soybean stems linear growth, the growth of aboveground mass and root system, and the chlorophylls and carotenoids accumulation during growing season.