Details

Autor(en) / Beteiligte

• Butler, Molly
• Breazeale, Garrett
• Mwangi, Eric
• Dowell, Elaine
• Dominguez, Samuel R
• Lamberth, Linda
• Hultén, Kristina G
• Jung, Sarah A
• Luethy, Paul M.

Titel

Development and validation of a multiplex real-time PCR assay for detection and quantification of Streptococcus pneumoniae in pediatric respiratory samples

Ist Teil von

• Microbiology spectrum, 2023-12, Vol.11 (6), p.e0211823-e0211823

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• ( ) is the world's leading cause of lower respiratory tract infection morbidity and mortality in children. However, current clinical microbiological methods have disadvantages. can be difficult to grow in laboratory conditions if a patient is pre-treated, and antigen testing has unclear clinical utility in children. Syndromic panel testing is less cost-effective than targeted PCR if clinical suspicion is high for a single pathogen. Also, such testing entails a full, expensive validation for each panel target if used for multiple respiratory sources. Therefore, better diagnostic modalities are needed. Our study validates a multiplex PCR assay with three genomic targets for semi-quantitative and quantitative molecular detection from lower respiratory sources for clinical testing and from upper respiratory sources for research investigation.