Details

Autor(en) / Beteiligte

• Cheng, Yinlong
• Li, Yining
• Xiong, Yonghong
• Zou, Yixin
• Chen, Siyu
• Zhang, Wenxiang
• Liu, Chang
• Shi, Yujie

Titel

Liver-specific knockdown of ANGPTL8 alters the structure of the gut microbiota in mice

Ist Teil von

• Annals of microbiology, 2020-09, Vol.70 (1), p.1-10, Article 58

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Purpose To investigate the effect of liver-specific knockdown of ANGPTL8 on the structure of the gut microbiota. Methods We constructed mice with liver-specific ANGPTL8 knockdown by using an adeno-associated virus serotype 8 (AAV8) system harbouring an ANGPTL8 shRNA. We analysed the structure and function of the gut microbiome through pyrosequencing and KEGG (Kyoto Encyclopedia of Genes and Genomes) functional prediction. Results Compared with controls, ANGPTL8 shRNA reduced the Simpson index and Shannon index ( p < 0.01) of the gut microbiota in mice. At the phylum level, the sh-ANGPTL8 group showed a healthier gut microbiota composition than controls ( Bacteroidetes : controls 67.52%, sh-ANGPTL8 80.75%; Firmicutes : controls 10.96%, sh-ANGPTL8 8.58%; Proteobacteria : controls 9.29%, sh-ANGPTL8 0.98%; F/B ratio: controls 0.16, sh-ANGPTL8 0.11). PCoA and UPGMA analysis revealed a significant difference in microbiota composition, while KEGG analysis revealed a significant difference in microbiota function between controls and the sh-ANGPTL8 group. Conclusion Our results revealed that inhibition of ANGPTL8 signalling altered the structure of the gut microbiome, which might further affect the metabolism of mice. We have thus identified ANGPTL8 as a novel hepatogenic hormone potentially involving the liver-gut axis and regulating the structure of the gut microbiota.