International journal of molecular sciences, 2018-10, Vol.19 (11), p.3327
2018
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- Autor(en) / Beteiligte
- Titel
- Protective Effects of Euphrasia officinalis Extract against Ultraviolet B-Induced Photoaging in Normal Human Dermal Fibroblasts
- Ist Teil von
- International journal of molecular sciences, 2018-10, Vol.19 (11), p.3327
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Ultraviolet (UV) radiation induces skin photoaging, which is associated with the elevation of matrix metalloproteinases (MMPs) and the impairment of collagen. The Euphrasia species play a well-known role in the treatment of certain eye disorders through their anti-oxidative and anti-inflammatory activities. However, their protective activity toward UVB-induced damage remains unclear. In the present study, we investigated the protective effect of (95% ethanol extract) on UVB-irradiated photoaging in normal human dermal fibroblasts (NHDFs). Our results show that extract exhibited obvious reactive oxygen species (ROS) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, enhanced NHDF cell migration, and reduced UVB-induced apoptosis. The UVB-induced increases in MMP-1 and MMP-3 and decrease in type I procollagen were ameliorated by treatment, which worked by suppressing the mitogen-activated protein kinase (MAPK) and nuclear transcription factor activator protein 1 (AP-1) signaling pathways. Taken together, our data strongly suggest that ethanol extract could reduce UVB-induced photoaging by alleviating oxidative stress, proinflammatory activity, and cell apoptosis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms19113327Titel-ID: cdi_doaj_primary_oai_doaj_org_article_50074719d8ee41439f21ab7282670dac
- Format
- –
- Schlagworte
- Acids, Activator protein 1, Antioxidants, Apoptosis, Cell adhesion & migration, Cell Movement - drug effects, Cell Movement - radiation effects, Cellular Senescence - drug effects, Cellular Senescence - radiation effects, Chromatography, Collagen, Collagen Type I - metabolism, Cytokines, Ethanol, Euphrasia, Euphrasia - chemistry, Euphrasia officinalis, Eye disorders, Fibroblasts, Fibroblasts - cytology, Fibroblasts - drug effects, Fibroblasts - radiation effects, Flavonoids, Inflammation, Interstitial collagenase, Investigations, Kinases, MAP kinase, Mass spectrometry, Matrix metalloproteinase, Matrix Metalloproteinase 1 - metabolism, Matrix Metalloproteinase 3 - metabolism, Matrix metalloproteinases, Oxidative stress, Phosphorylation, photoaging, Plant Extracts - pharmacology, Procollagen, Protein kinase, Proteins, Radiation, Scavenging, Scientific imaging, Signal transduction, Skin - cytology, Skin - drug effects, Skin - radiation effects, Skin Aging - drug effects, Skin Aging - radiation effects, Stromelysin 1, Sulfonic acid, Transcription factors, type I procollagen, ultraviolet B, Ultraviolet radiation, Ultraviolet Rays