Details

Autor(en) / Beteiligte

• Yaffe, Zak A.
• Ding, Shilei
• Sung, Kevin
• Chohan, Vrasha
• Marchitto, Lorie
• Doepker, Laura
• Ralph, Duncan
• Nduati, Ruth
• Matsen, Frederick A.
• Finzi, Andrés
• Overbaugh, Julie

Titel

Reconstruction of a polyclonal ADCC antibody repertoire from an HIV-1 non-transmitting mother

Ist Teil von

• iScience, 2023-05, Vol.26 (5), p.106762-106762, Article 106762

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Human natural history and vaccine studies support a protective role of antibody dependent cellular cytotoxicity (ADCC) activity against many infectious diseases. One setting where this has consistently been observed is in HIV-1 vertical transmission, where passively acquired ADCC activity in HIV-exposed infants has correlated with reduced acquisition risk and reduced pathogenesis in HIV+ infants. However, the characteristics of HIV-specific antibodies comprising a maternal plasma ADCC response are not well understood. Here, we reconstructed monoclonal antibodies (mAbs) from memory B cells from late pregnancy in mother MG540, who did not transmit HIV to her infant despite several high-risk factors. Twenty mAbs representing 14 clonal families were reconstructed, which mediated ADCC and recognized multiple HIV Envelope epitopes. In experiments using Fc-defective variants, only combinations of several mAbs accounted for the majority of plasma ADCC of MG540 and her infant. We present these mAbs as evidence of a polyclonal repertoire with potent HIV-directed ADCC activity. [Display omitted] •ADCC-mediating antibodies were isolated from an HIV-infected non-transmitting mother•Multiple antibodies contributed to the plasma ADCC of this individual•The ADCC antibody repertoire in maternal and early infant plasma is similar•These findings suggest plasma ADCC in this mother is because of a polyclonal response Immunology; Pediatrics; Virology