Details

Autor(en) / Beteiligte

• Bencsik, Péter
• Kiss, Krisztina
• Ágg, Bence
• Baán, Júlia A
• Ágoston, Gergely
• Varga, Albert
• Gömöri, Kamilla
• Mendler, Luca
• Faragó, Nóra
• Zvara, Ágnes
• Sántha, Péter
• Puskás, László G
• Jancsó, Gábor
• Ferdinandy, Péter

Titel

Sensory Neuropathy Affects Cardiac miRNA Expression Network Targeting IGF-1 , SLC2a-12 , EIF-4e , and ULK-2 mRNAs

Ist Teil von

• International journal of molecular sciences, 2019-02, Vol.20 (4), p.991

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2019

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Here we examined myocardial microRNA (miRNA) expression profile in a sensory neuropathy model with cardiac diastolic dysfunction and aimed to identify key mRNA molecular targets of the differentially expressed miRNAs that may contribute to cardiac dysfunction. Male Wistar rats were treated with vehicle or capsaicin for 3 days to induce systemic sensory neuropathy. Seven days later, diastolic dysfunction was detected by echocardiography, and miRNAs were isolated from the whole ventricles. Out of 711 known miRNAs measured by miRNA microarray, the expression of 257 miRNAs was detected in the heart. As compared to vehicle-treated hearts, , , , , , , and were downregulated, while was upregulated as validated also by quantitative real time polymerase chain reaction (qRT-PCR). By an in silico network analysis, we identified common mRNA targets (insulin-like growth factor 1 ( ), solute carrier family 2 facilitated glucose transporter member 12 ( ), eukaryotic translation initiation factor 4e ), and Unc-51 like autophagy activating kinase 2 ( )) targeted by at least three altered miRNAs. Predicted upregulation of these mRNA targets were validated by qRT-PCR. This is the first demonstration that sensory neuropathy affects cardiac miRNA expression network targeting , , , and , which may contribute to cardiac diastolic dysfunction. These results further support the need for unbiased omics approach followed by in silico prediction and validation of molecular targets to reveal novel pathomechanisms.