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Genomic Characterization of Prevalent mcr-1, mcr-4, and mcr-5 Escherichia coli Within Swine Enteric Colibacillosis in Spain
Ist Teil von
Frontiers in microbiology, 2019-11, Vol.10, p.2469-2469
Ort / Verlag
Frontiers Media S.A
Erscheinungsjahr
2019
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
Antimicrobial agents are crucial for the treatment of many bacterial diseases in pigs, however, the massive use of critically important antibiotics such as colistin, fluoroquinolones and 3rd–4th-generation cephalosporins often selects for co-resistance. Based on a comprehensive characterization of 35 colistin-resistant
Escherichia coli
from swine enteric colibacillosis, belonging to prevalent Spanish lineages, the aims of the present study were to investigate the characteristics of
E. coli
clones successfully spread in swine and to assess the correlation of the
in vitro
results with
in silico
predictions from WGS data. The resistome analysis showed six different
mcr
variants:
mcr-1.1
;
mcr-1.10
;
mcr-4.1
;
mcr-4.2
;
mcr-4.5;
and
mcr-5.1
. Additionally,
bla
CTX–M–
14
,
bla
CTX–M–
32
and
bla
SHV–
12
genes were present in seven genomes. PlasmidFinder revealed that
mcr-1.1
genes located mainly on IncHI2 and IncX4 types, and
mcr-4
on ColE10-like plasmids. Twenty-eight genomes showed a
gyrA
S83L substitution, and 12 of those 28 harbored double-serine mutations
gyrA
S83L and
parC
S80I, correlating with
in vitro
quinolone-resistances. Notably, 16 of the 35
mcr-
bearing genomes showed mutations in the PmrA (S39I) and PmrB (V161G) proteins. The summative presence of mechanisms, associated with high-level of resistance to quinolones/fluoroquinolones and colistin, could be conferring adaptive advantages to prevalent pig
E. coli
lineages, such as the ST10-A (CH11-24), as presumed for ST131. SerotypeFinder allowed the H-antigen identification of
in vitro
non-mobile (HNM) isolates, revealing that 15 of the 21 HNM
E. coli
analyzed were H39. Since the H39 is associated with the most prevalent O antigens worldwide within swine colibacillosis, such as O108 and O157, it would be probably playing a role in porcine colibacillosis to be considered as a valuable subunit antigen in the formulation of a broadly protective Enterotoxigenic
E. coli
(ETEC) vaccine. Our data show common features with other European countries in relation to a prevalent clonal group (CC10), serotypes (O108:H39, O138:H10, O139:H1, O141:H4), high plasmid content within the isolates and
mcr
location, which would support global alternatives to the use of antibiotics in pigs. Here, we report for first time a rare finding so far, which is the co-occurrence of double colistin-resistance mechanisms in a significant number of
E. coli
isolates.