Details

Autor(en) / Beteiligte

• Petrilli, Jéssica D.
• Araújo, Luana E.
• da Silva, Luciane Sussuchi
• Laus, Ana Carolina
• Müller, Igor
• Reis, Rui Manuel
• Netto, Eduardo Martins
• Riley, Lee W.
• Arruda, Sérgio
• Queiroz, Adriano

Titel

Whole blood mRNA expression-based targets to discriminate active tuberculosis from latent infection and other pulmonary diseases

Ist Teil von

• Scientific reports, 2020-12, Vol.10 (1), p.22072-22072, Article 22072

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Current diagnostic tests for tuberculosis (TB) are not able to predict reactivation disease progression from latent TB infection (LTBI). The main barrier to predicting reactivation disease is the lack of our understanding of host biomarkers associated with progression from latent infection to active disease. Here, we applied an immune-based gene expression profile by NanoString platform to identify whole blood markers that can distinguish active TB from other lung diseases (OPD), and that could be further evaluated as a reactivation TB predictor. Among 23 candidate genes that differentiated patients with active TB from those with OPD, nine genes (CD274, CEACAM1, CR1, FCGR1A/B, IFITM1, IRAK3, LILRA6, MAPK14, PDCD1LG2) demonstrated sensitivity and specificity of 100%. Seven genes (C1QB, C2, CCR2, CCRL2, LILRB4, MAPK14, MSR1) distinguished TB from LTBI with sensitivity and specificity between 82 and 100%. This study identified single gene candidates that distinguished TB from OPD and LTBI with high sensitivity and specificity (both > 82%), which may be further evaluated as diagnostic for disease and as predictive markers for reactivation TB.