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Decay of Enterococcus faecalis, Vibrio cholerae and MS2 Coliphage in a Laboratory Mesocosm Under Brackish Beach Conditions
Ist Teil von
Frontiers in public health, 2019-09, Vol.7, p.269-269
Ort / Verlag
Frontiers Research Foundation
Erscheinungsjahr
2019
Quelle
EZB Free E-Journals
Beschreibungen/Notizen
Enterococci are fecal indicator bacteria (FIB) used for monitoring the microbial quality of bathing water. However, the reliability of health protection by monitoring FIB is questioned. This study evaluated the decay pattern of
Enterococcus faecalis
in beach environment and compared it with decay patterns of the pathogen
Vibrio cholerae
and the virus indicator MS2 coliphage. Tests were done in an experimental mesocosm simulating natural Nordic summer daylight conditions with and without the aquatic plant
Myriophyllum sibiricum
. The decay of the spiked test microbes (
E. faecalis, V. cholera
, and MS2) was enumerated up to 27 days from two coastal bathing water mesocosms with subtidal sediment.
E. faecalis
and
V. cholerae
exhibited non-linear biphasic decay patterns and were detected upmost toward the end of the experiment in the water, sediment, and vegetation. The gene copies of
V. cholerae
dropped to a minimum by days 6–8 but then the numbers increased back up to nearly the spiked level. The MS2 coliphage was detected only up to 8–10 days into the experiment solely in the water where a log-linear decay pattern was seen. The test microbe, sample origin (water, sediment or vegetation) and, as determined for
E. faecalis
, the enumeration method (culture-based or qPCR) affected the decay pattern.
E. faecalis
decayed faster in water than in sediment and vegetation. Positive correlations between culturable
E. faecalis
counts with
V. cholerae
gene copies and MS2 counts were recorded on the first few days of the experiment. This study demonstrated the important role of water, sediment and vegetation regarding the partitioning of pathogens and fecal indicators in coastal environment. The enumeration of the enterococci counts alone was not sufficient for predicting the numbers of pathogens such as
Vibrio
spp. in bathing water. The growth of
Vibrio
spp. in the Baltic Sea deserves more attention and might require water quality monitoring to be applied for these pathogens in the coming years due to the predicted rise in sea surface temperature favoring
Vibrio
spp. growth. Further, different decay patterns observed between MS2 and enterococci emphasize the need for and importance of a viral indicator in assessing water quality more comprehensively.