Details

Autor(en) / Beteiligte

• de Souza, Aline Morais
• Real, Caroline Cristiano
• Junqueira, Mara de Souza
• Estessi de Souza, Larissa
• Navarro Marques, Fábio Luiz
• Buchpiguel, Carlos Alberto
• Chammas, Roger
• Sapienza, Marcelo Tatit
• de Paula Faria, Daniele

Titel

Potential of [ 11 C]( R )-PK11195 PET Imaging for Evaluating Tumor Inflammation: A Murine Mammary Tumor Model

Ist Teil von

• Pharmaceutics, 2022-12, Vol.14 (12), p.2715

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2022

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Breast tumor inflammation is an immunological process that occurs mainly by mediation of Tumor-Associated Macrophages (TAM). Aiming for a specific measurement of tumor inflammation, the current study evaluated the potential of Positron Emission Tomography (PET) imaging with [ C]( )-PK11195 to evaluate tumor inflammation in a mammary tumor animal model. Female Balb/C mice were inoculated with 4T1 cells. The PET imaging with [ C]( )-PK11195 and [ F]FDG was acquired 3 days, 1 week, and 2 weeks after cell inoculation. The [ C]( )-PK11195 tumor uptake increased from 3 days to 1 week, and decreased at 2 weeks after cell inoculation, as opposed to the [ F]FDG uptake, which showed a slight decrease in uptake at 1 week and increased uptake at 2 weeks. In the control group, no significant differences occurred in tracer uptake over time. Tumor uptake of both radiopharmaceuticals is more expressed in tumor edge regions, with greater intensity at 2 weeks, as demonstrated by [ C]( )-PK11195 autoradiography and immunofluorescence with TSPO antibodies and CD86 pro-inflammatory phenotype. The [ C]( )-PK11195 was able to identify heterogeneous tumor inflammation in a murine model of breast cancer and the uptake varied according to tumor size. Together with the glycolytic marker [ F]FDG, molecular imaging with [ C]( )-PK11195 may provide a better characterization of inflammatory responses in cancer.