Details

Autor(en) / Beteiligte

• Yuan, Renyikun
• Liu, Zhenjie
• Zhao, Jianping
• Wang, Qin-Qin
• Zuo, Aiyuan
• Huang, Liting
• Gao, Hongwei
• Xu, Qiongming
• Khan, Ikhlas A.
• Yang, Shilin

Titel

Novel compounds in fruits of coriander (Coşkuner & Karababa) with anti-inflammatory activity

Ist Teil von

• Journal of functional foods, 2020-10, Vol.73, p.104145, Article 104145

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2020

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• [Display omitted] •Four new compounds were identified in Coriander (Coriandrum Sativum L.).•Compound 3 exhibited a significant anti-inflammatory effect.•Compound 3 decreased inflammatory response in cells.•NF-κB and MAPKs pathways were involved in compound 3′s anti-inflammatory process. Coriander, Coriandrum Sativum L., is one of the commonest food and medicinal plants in many countries, but its chemical ingredients and pivotal role in anti-inflammatory activity have not been fully explored. The present study aimed to identify new compounds in the fruits of coriander and explore their anti-inflammatory activity. The compounds were isolated by chromatographic seperations and identified using spectroscopic and spectrometric methods. RAW264.7 macrophage cells were used to detect the anti-inflammatory activity of the compounds via Griess assay, western blotting, ELISA, and flow cytometry methods. The study resulted in the discovery of four new compounds, which were identified as: 4α-(furo[2,3-d]pyrimidin-6′-ylmethyl)-9α-propylnonolactone (1), 4-(formyloxy)-4-(6′-methylcyclohex-1-en-1-yl)butanoate(2), (7α,8α)-3α-hydroxyl-12,13α-dimethyl-5(6)-en-bicyclo[5,3,0]caprolactone (3), 7-methoxy-4-methyl-5,6-dihydro-7H-(2-hydroxypropan-2-yl)furo[2,3-f] coumarin (4). Compound 3 showed the highest anti-inflammatory activity with IC50 of 6.25 μM for an inhibitory effect on nitrite oxide (NO) level. In addition, compound 3 decreased the lipopolysaccharides-stimulated generations of ROS and the inflammatory cytokines (IL-6 and TNF-α). Mechanism exploration indicated that compound 3 suppressed inflammatory mediators’ expression, like iNOS and COX-2. Furthermore, the NF-κB and MAPK pathways were involved in the anti-inflammatory process of compound 3.