Details

Autor(en) / Beteiligte

• Queiroux, Clothilde
• Bonnet, Muriel
• Saraoui, Taous
• Delpech, Pierre
• Veisseire, Philippe
• Rifa, Etienne
• Moussard, Cécile
• Gagne, Geneviève
• Delbès, Céline
• Bornes, Stéphanie

Titel

Dialogue between Staphylococcus aureus SA15 and Lactococcus garvieae strains experiencing oxidative stress

Ist Teil von

• BMC microbiology, 2018-11, Vol.18 (1), p.193-11, Article 193

Ort / Verlag

England: BioMed Central Ltd

Erscheinungsjahr

2018

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Staphylococcus aureus is an important foodborne pathogen. Lactococcus garvieae is a lactic acid bacterium found in dairy products; some of its strains are able to inhibit S. aureus growth by producing H O . Three strains of L. garvieae from different origins were tested for their ability to inhibit S. aureus SA15 growth. Two conditions were tested, one in which H O was produced (high aeration) and another one in which it was not detected (low aeration). Several S. aureus genes related to stress, H O -response and virulence were examined in order to compare their level of expression depending on the inoculated L. garvieae strain. Simultaneous L. garvieae H O metabolism gene expression was followed. The results showed that under high aeration condition, L. garvieae strains producing H O (N201 and CL-1183) inhibited S. aureus SA15 growth and impaired its ability to deal with hydrogen peroxide by repressing H O -degrading genes. L. garvieae strains induced overexpression of S. aureus stress-response genes while cell division genes and virulence genes were repressed. A catalase treatment partially or completely restored the SA15 growth. In addition, the H O non-producing L. garvieae strain (Lg2) did not cause any growth inhibition. The SA15 stress-response genes were down-regulated and cell division genes expression was not affected. Under low aeration condition, while none of the strains tested exhibited H O -production, the 3 L. garvieae strains inhibited S. aureus SA15 growth, but to a lesser extent than under high aeration condition. Taken together, these results suggest a L. garvieae strain-specific anti-staphylococcal mechanism and an H O involvement in at least two of the tested L. garvieae strains.