Details

Autor(en) / Beteiligte

• Chen, Ming-Jer
• Hsu, An
• Lin, Pei-Yi
• Chen, Yu-Ling
• Wu, Ko-Wen
• Chen, Kuan-Chun
• Wang, Tiffany
• Yi, Yu-Chiao
• Kung, Hsiao-Fan
• Chang, Jui-Chun
• Yang, Wen-Jui
• Lu, Farn
• Guu, Hwa-Fen
• Chen, Ya-Fang
• Chuan, Shih-Ting
• Chen, Li-Yu
• Chen, Ching-Hung
• Yang, Pok Eric
• Huang, Jack Yu-Jen

Titel

Development of a Predictive Model for Optimization of Embryo Transfer Timing Using Blood-Based microRNA Expression Profile

Ist Teil von

• International journal of molecular sciences, 2023-12, Vol.25 (1), p.76

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• MicroRNAs (miRNAs) can regulate the expression of genes involved in the establishment of the window of implantation (WOI) in the endometrium. Recent studies indicated that cell-free miRNAs in uterine fluid and blood samples could act as alternative and non-invasive sample types for endometrial receptivity analysis. In this study, we attempt to systematically evaluate whether the expression levels of cell-free microRNAs in blood samples could be used as non-invasive biomarkers for assessing endometrial receptivity status. We profiled the miRNA expression levels of 111 blood samples using next-generation sequencing to establish a predictive model for the assessment of endometrial receptivity status. This model was validated with an independent dataset ( = 73). The overall accuracy is 95.9%. Specifically, we achieved accuracies of 95.9%, 95.9%, and 100.0% for the pre-receptive group, the receptive group, and the post-respective group, respectively. Additionally, we identified a set of differentially expressed miRNAs between different endometrial receptivity statuses using the following criteria: -value < 0.05 and fold change greater than 1.5 or less than -1.5. In conclusion, the expression levels of cell-free miRNAs in blood samples can be utilized in a non-invasive manner to distinguish different endometrial receptivity statuses.