Stem cell research & therapy, 2023-08, Vol.14 (1), p.197-197, Article 197
2023
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- Autor(en) / Beteiligte
- Titel
- Mesenchymal stem cells shift the pro-inflammatory phenotype of neutrophils to ameliorate acute lung injury
- Ist Teil von
- Stem cell research & therapy, 2023-08, Vol.14 (1), p.197-197, Article 197
- Ort / Verlag
- England: BioMed Central Ltd
- Erscheinungsjahr
- 2023
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- Mesenchymal stem cell (MSC) treatment plays a major role in the management of acute lung injury (ALI), and neutrophils are the initial line of defense against ALI. However, the effect of MSCs on neutrophils in ALI remains mostly unknown. We investigated the characteristics of neutrophils in lung tissue of ALI mice induced by lipopolysaccharide after treatment with MSCs using single-cell RNA sequencing. Neutrophils separated from lung tissue in ALI were co-cultured with MSCs, and then samples were collected for reverse transcription-polymerase chain reaction and flow cytometry. During inflammation, six clusters of neutrophils were identified, annotated as activated, aged, and circulatory neutrophils. Activated neutrophils had higher chemotaxis, reactive oxygen species (ROS) production, and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase scores than aged neutrophils. Circulatory neutrophils occurred mainly in healthy tissue and were characterized by higher expression of Cxcr2 and Sell. Activated neutrophils tended to exhibit higher expression of Cxcl10 and Cd47, and lower expression of Cd24a, while aged neutrophils expressed a lower level of Cd47 and higher level of Cd24a. MSC treatment shifted activated neutrophils toward an aged neutrophil phenotype by upregulating the expression of CD24, thereby inhibiting inflammation by reducing chemotaxis, ROS production, and NADPH oxidase. We identified the immunosuppressive effects of MSCs on the subtype distribution of neutrophils and provided new insight into the therapeutic mechanism of MSC treatment in ALI.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1757-6512eISSN: 1757-6512DOI: 10.1186/s13287-023-03438-wTitel-ID: cdi_doaj_primary_oai_doaj_org_article_45dc2df242f84e8589ae54a6872006ca
- Format
- –
- Schlagworte
- Acute lung injury, Acute Lung Injury - chemically induced, Animals, CD47 Antigen - metabolism, Chemotaxis, Clinical trials, CXCL10 protein, CXCR2 protein, Cytokines, Data processing, Dimensional analysis, Flow cytometry, Genetic aspects, Genomics, Inflammation, Inflammation - metabolism, Inflammation - therapy, Lavage, Leukocytes (neutrophilic), Lipopolysaccharides, Lipopolysaccharides - toxicity, Lung - metabolism, Lung diseases, Lungs, Mesenchymal stem cell, Mesenchymal Stem Cell Transplantation, Mesenchymal stem cells, Mesenchymal Stem Cells - metabolism, Mice, Mouse model, NAD(P)H oxidase, NADPH-diaphorase, Neutrophil, Neutrophils, Neutrophils - metabolism, Niacinamide, Oxidases, Phenotypes, Phosphates, Reactive oxygen species, Reactive Oxygen Species - metabolism, Reverse transcription, RNA, RNA sequencing, Single-cell RNA sequencing, Software packages, Stem cells