Molecules (Basel, Switzerland), 2021-12, Vol.27 (1), p.199
2021
Details
- Autor(en) / Beteiligte
- Titel
- 25-Hydroxycholesterol-Induced Oxiapoptophagy in L929 Mouse Fibroblast Cell Line
- Ist Teil von
- Molecules (Basel, Switzerland), 2021-12, Vol.27 (1), p.199
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- 25-hydroxycholesterol (25-HC) is an oxysterol synthesized from cholesterol by cholesterol-25-hydroxylase during cholesterol metabolism. The aim of this study was to verify whether 25-HC induces oxiapoptophagy in fibroblasts. 25-HC not only decreased the survival of L929 cells, but also increased the number of cells with condensed chromatin and altered morphology. Fluorescence-activated cell sorting results showed that there was a dose-dependent increase in the apoptotic populations of L929 cells upon treatment with 25-HC. 25-HC-induced apoptotic cell death was mediated by the death receptor-dependent extrinsic and mitochondria-dependent intrinsic apoptosis pathway, through the cascade activation of caspases including caspase-8, -9, and -3 in L929 cells. There was an increase in the levels of reactive oxygen species and inflammatory mediators such as inducible nitric oxide synthase, cyclooxygenase-2, nitric oxide, and prostaglandin E2 in L929 cells treated with 25-HC. Moreover, 25-HC caused an increase in the expression of beclin-1 and microtubule-associated protein 1A/1B-light chain 3, an autophagy biomarker, in L929 cells. There was a significant decrease in the phosphorylation of protein kinase B (Akt) in L929 cells treated with 25-HC. Taken together, 25-HC induced oxiapoptophagy through the modulation of Akt and p53 cellular signaling pathways in L929 cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1420-3049eISSN: 1420-3049DOI: 10.3390/molecules27010199Titel-ID: cdi_doaj_primary_oai_doaj_org_article_441db17e7ff54fd1ae26fc9ae27603c9
- Format
- –
- Schlagworte
- 25-hydroxycholesterol, AKT protein, Alzheimer's disease, Animals, Apoptosis, Apoptosis - drug effects, Autophagy, Autophagy - drug effects, Biomarkers, Caspase-8, Cell death, Cell Death - drug effects, Cell Line, Cell survival, Cell Survival - drug effects, Cholesterol, Chromatin, Cyclooxygenase-2, Cytology, Cytotoxicity, Fibroblasts, Fibroblasts - drug effects, Fibroblasts - metabolism, Flow cytometry, Fluorescence, Hydroxycholesterols - chemistry, Hydroxycholesterols - pharmacology, Hydroxylase, Inflammation, Inflammation Mediators - metabolism, Ligands, Lipid metabolism, Lipids, Lymphoma, Metabolism, Mice, Microtubule-associated protein 1, Mitochondria, Molecular Structure, Nitric oxide, Nitric-oxide synthase, oxiapoptophagy, Oxidative stress, Oxidative Stress - drug effects, oxysterol, p53 Protein, Phosphorylation, Physiology, Prostaglandin E2, Proteins, Reactive Oxygen Species - metabolism, Signal transduction, Sterols