Details

Autor(en) / Beteiligte

• Andersen, Peter
• Tampakakis, Emmanouil
• Jimenez, Dennisse V.
• Kannan, Suraj
• Miyamoto, Matthew
• Shin, Hye Kyung
• Saberi, Amir
• Murphy, Sean
• Sulistio, Edrick
• Chelko, Stephen P.
• Kwon, Chulan

Titel

Precardiac organoids form two heart fields via Bmp/Wnt signaling

Ist Teil von

• Nature communications, 2018-08, Vol.9 (1), p.3140-13, Article 3140

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• The discovery of the first heart field (FHF) and the second heart field (SHF) led us to understand how cardiac lineages and structures arise during development. However, it remains unknown how they are specified. Here, we generate precardiac spheroids with pluripotent stem cells (PSCs) harboring GFP/RFP reporters under the control of FHF/SHF markers, respectively. GFP + cells and RFP + cells appear from two distinct areas and develop in a complementary fashion. Transcriptome analysis shows a high degree of similarities with embryonic FHF/SHF cells. Bmp and Wnt are among the most differentially regulated pathways, and gain- and loss-of-function studies reveal that Bmp specifies GFP + cells and RFP + cells via the Bmp/Smad pathway and Wnt signaling, respectively. FHF/SHF cells can be isolated without reporters by the surface protein Cxcr4. This study provides novel insights into understanding the specification of two cardiac origins, which can be leveraged for PSC-based modeling of heart field/chamber-specific disease. The heart arises from distinct progenitor cells of both the first and second heart fields (FHF and SHF). Here, the authors generated precardiac organoids from mouse and human pluripotent cells and show that FHF and SHF cells form similarly to their in vivo counterparts in response to BMP and Wnt signalling, respectively.