Details

Autor(en) / Beteiligte

• Rivera-Torruco, Guadalupe
• Martínez-Mendiola, Carolina A.
• Angeles-Floriano, Tania
• Jaimes-Ortega, Gustavo Alberto
• Maravillas-Montero, José Luis
• García-Contreras, Rodolfo
• González, Yolanda
• Juárez, Esmeralda
• Nava, Porfirio
• Ortiz-Navarrete, Vianney
• Medina-Contreras, Oscar
• Licona-Limón, Paula
• Valle-Rios, Ricardo
• Cavaliere, Carlo
• Carlo Cavaliere

Titel

Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1+ Hematopoietic Stem-Like Cells in Homeostasis and during Infection

Ist Teil von

• Journal of immunology research, 2022-04, Vol.2022, p.2909487-13

Ort / Verlag

Egypt: Hindawi

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The process by which blood cells are generated has been widely studied in homeostasis and during pathogen-triggered inflammatory response. Recently, murine lungs have been shown to be a significant source of hematopoietic progenitors in a process known as extramedullary hematopoiesis. Using multiparametric flow cytometry, we have identified mesenchymal, endothelial, and hematopoietic progenitor cells that express the secreted small protein Isthmin 1 (ISM1). Further characterization of hematopoietic progenitor cells indicated that ISM1+ Lineage- Sca-1+ c-kit+ (ISM1+ LSK) cells are enriched in short-term hematopoietic stem cells (ST-HSCs). Moreover, most Sca-1+ ISM1+ cells express the residence marker CD49a, and this correlated with their localization in the extravascular region of the lung, indicating that ISM1+ cells are lung-resident cells. We also observed that ISM1+ cells express TLR4, TLR5, and TLR9, and, in a mouse model of sepsis induced by P. aeruginosa, we observed that all the LSK and ISM1+LSK cells were affected. We conclude that ISM1 is a novel biomarker associated with progenitor-like cells. ISM1+ cells are involved in the response to a bacterial challenge, suggesting an association between ISM1-producing cells and dangerous inflammatory responses like sepsis.