Details

Autor(en) / Beteiligte

• Yu, Nam-Kyung
• McClatchy, Daniel B.
• Diedrich, Jolene K.
• Romero, Sarah
• Choi, Jun-Hyeok
• Martínez-Bartolomé, Salvador
• Delahunty, Claire M.
• Muotri, Alysson R.
• Yates, John R.

Titel

Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells

Ist Teil von

• iScience, 2021-11, Vol.24 (11), p.103321-103321, Article 103321

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A single protein can be multifaceted depending on the cellular contexts and interacting molecules. LIN28A is an RNA-binding protein that governs developmental timing, cellular proliferation, differentiation, stem cell pluripotency, and metabolism. In addition to its best-known roles in microRNA biogenesis, diverse molecular roles have been recognized. In the nervous system, LIN28A is known to play critical roles in proliferation and differentiation of neural progenitor cells (NPCs). We profiled the endogenous LIN28A-interacting proteins in NPCs differentiated from human induced pluripotent stem (iPS) cells using immunoprecipitation and liquid chromatography-tandem mass spectrometry. We identified over 500 LIN28A-interacting proteins, including 156 RNA-independent interactors. Functions of these proteins span a wide range of gene regulatory processes. Prompted by the interactome data, we revealed that LIN28A may impact the subcellular distribution of its interactors and stress granule formation upon oxidative stress. Overall, our analysis opens multiple avenues for elaborating molecular roles and characteristics of LIN28A. [Display omitted] •IP-MS identified >500 LIN28A-interacting proteins from neural progenitor cells•Functions of LIN28A-associated proteins span a wide range of gene regulatory processes•LIN28A impacts subcellular distribution of EIF3D•LIN28A interferes with SG formation upon oxidative stress Gene network; Molecular neuroscience; Omics; Stem cells research