Details

Autor(en) / Beteiligte

• van Kleeff, Paula J M
• Mastop, Marieke
• Sun, Pulu
• Dangol, Sarmina
• van Doore, Eva
• Dekker, Henk L
• Kramer, Gertjan
• Lee, Soohyun
• Ryu, Choong-Min
• de Vos, Martin
• Schuurink, Robert C

Titel

Discovery of Three Bemisia tabaci Effectors and Their Effect on Gene Expression in Planta

Ist Teil von

• Molecular plant-microbe interactions, 2024-04, Vol.37 (4), p.380-395

Ort / Verlag

United States: American Phytopathological Society

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• (whitefly) is a polyphagous agroeconomic pest species complex. Two members of this species complex, Mediterranean (MED) and Middle-East-Asia Minor 1 (MEAM1), have a worldwide distribution and have been shown to manipulate plant defenses through effectors. In this study, we used three different strategies to identify three MEAM1 proteins that can act as effectors. Effector B1 was identified using a bioinformatics-driven effector-mining strategy, whereas effectors S1 and P1 were identified in the saliva of whiteflies collected from artificial diet and in phloem exudate of tomato on which nymphs were feeding, respectively. These three effectors were specific and able to increase whitefly fecundity when transiently expressed in tobacco plants ( ). Moreover, they reduced growth of pv. in . All three effectors changed gene expression in planta, and B1 and S1 also changed phytohormone levels. Gene ontology and KEGG pathway enrichment analysis pinpointed plant-pathogen interaction and photosynthesis as the main enriched pathways for all three effectors. Our data thus show the discovery and validation of three new MEAM1 effectors that increase whitefly fecundity and modulate plant immunity. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.