Nature communications, 2019-11, Vol.10 (1), p.5080-12, Article 5080
2019
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Designing yeast as plant-like hyperaccumulators for heavy metals
- Ist Teil von
- Nature communications, 2019-11, Vol.10 (1), p.5080-12, Article 5080
- Ort / Verlag
- London: Nature Publishing Group UK
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Hyperaccumulators typically refer to plants that absorb and tolerate elevated amounts of heavy metals. Due to their unique metal trafficking abilities, hyperaccumulators are promising candidates for bioremediation applications. However, compared to bacteria-based bioremediation systems, plant life cycle is long and growing conditions are difficult to maintain hindering their adoption. Herein, we combine the robust growth and engineerability of bacteria with the unique waste management mechanisms of plants by using a more tractable platform-the common baker’s yeast-to create plant-like hyperaccumulators. Through overexpression of metal transporters and engineering metal trafficking pathways, engineered yeast strains are able to sequester metals at concentrations 10–100 times more than established hyperaccumulator thresholds for chromium, arsenic, and cadmium. Strains are further engineered to be selective for either cadmium or strontium removal, specifically for radioactive Sr 90 . Overall, this work presents a systematic approach for transforming yeast into metal hyperaccumulators that are as effective as their plant counterparts. Existing heavy metal bioremediation systems are mainly based on plants, which require long growing time in specific conditions. Here, the authors mimic the characteristics of plant hyperaccumulators to engineer more tractable baker’s yeast and achieve 10–100-fold higher accumulation of chromium, arsenic, or cadmium.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-019-13093-6Titel-ID: cdi_doaj_primary_oai_doaj_org_article_3fdfed0b8b1543ccb8e167e9d45c1134
- Format
- –
- Schlagworte
- 38/23, 38/70, 49, 631/61/168, 631/61/252/58, 631/61/318, 82/80, Antiporters - genetics, Antiporters - metabolism, Arsenic, Arsenic - metabolism, Bacteria, Biodegradation, Environmental, Bioremediation, Cadmium, Cadmium - metabolism, Carrier Proteins - genetics, Carrier Proteins - metabolism, Cation Transport Proteins - genetics, Cation Transport Proteins - metabolism, Chromium, Chromium - metabolism, Copper Transport Proteins - genetics, Copper Transport Proteins - metabolism, Copper Transporter 1 - genetics, Copper Transporter 1 - metabolism, Heavy metals, Humanities and Social Sciences, Iron-Binding Proteins - genetics, Iron-Binding Proteins - metabolism, Life cycles, Membrane Transport Proteins - genetics, Membrane Transport Proteins - metabolism, Metabolic Engineering - methods, Metal concentrations, Metals, Heavy - metabolism, multidisciplinary, Saccharomyces cerevisiae - genetics, Saccharomyces cerevisiae - metabolism, Saccharomyces cerevisiae Proteins - genetics, Saccharomyces cerevisiae Proteins - metabolism, Science, Science (multidisciplinary), SLC31 Proteins - genetics, SLC31 Proteins - metabolism, Strains (organisms), Strontium, Strontium - metabolism, Strontium Radioisotopes - metabolism, Waste management, Yeast, Yeasts