Details

Autor(en) / Beteiligte

• Ma, Junxing
• Chen, Yaoxing
• Wang, Zixu
• Wang, Ran
• Dong, Yulan

Titel

Lactiplantibacillus plantarum CR12 attenuates chronic unforeseeable mild stress induced anxiety and depression-like behaviors by modulating the gut microbiota-brain axis

Ist Teil von

• Journal of functional foods, 2023-08, Vol.107, p.105710, Article 105710

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2023

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• In conclusion, CUMS can lead to changes in the microglial morphology and the secretion of inflammatory factors, resulting inflammation in the hippocampus and leading to cognitive impairment and depressive-like behavior. The use of Lactiplantibacillus plantarum CR12 can prevent the depressive-like behavior and cognitive impairment, which may be related to its ability to regulate inflammatory function of microglia by butyrate, a metabolite of gut microbiota, and protect against the neurodevelopmental deficits. These results demonstrate that gut microbiota and their SCFAs metabolites play important roles in chronic stress disorder. Therefore, methods that modulate the gut microbiome and its metabolites may be a promising treatment strategy for depression. [Display omitted] •The role of 5-HT in chronic stress cognitive impairment through intestinal microbiota.•disturbance.•Lactiplantibacillus plantarum CR12 effectively restored intestinal flora homeostasis by.•restoring flora abundance and thereby increased intestinal short-chain fatty acid.•(SCFAs) content.•Lactiplantibacillus plantarum CR12 (which promote 5-HT concentration) may alleviate rodent depressive and anxiety-like behaviors on chronic stress-induced cognitive impairment in mice and its mechanisms of action were demonstrated by analyzing the correlations between the above indicators and the cognitive memory and depression behavior of the mice. Depression is a progressive neurodegenerative disease characterized by cognitive deficits and psychiatric symptoms. The gut microbiota-brain axis plays a pivotal role during depression development. CUMS mice and CUMS+FLX mice were used in the study. Finally, Lactiplantibacillus plantarum (L. plantarum) CR12 powder group were orally administrated to mice with CUMS. We used Open field test, Morris water maze, Tail suspension test and Sucrose preference test to examine learning related behaviors. In addition, Immunofluorescence, ELISA, qPCR, Western blot and Microbial sequencing were employed to address Cognitive and behavioral disorders in CUMS mice. We found that treatment of L. plantarum CR12 significantly improved cognitive function and spatial memory of Open field test, Tail suspension test and Sucrose preference, accompanied by attenuated the anxiety- and obsessive-like behaviors in the CUMS depression mice model and increased hippocampal autophagy levels, decreased neurotransmitter levels. Notably, L. plantarum CR12 re-constructed the gut microbiota composition, including increasing the relative abundance of Lactobacillus and reducing the relative abundance of Helicobacter pylori (H. pylori), and enhanced the butyrate formation, as well as related microbes levels. In conclusion, the present study indicated that L. plantarum CR12 significantly attenuates the cognitive and mental deficits in the CUMS mice, which could be partly explained by the reshaped microbiome and enhanced SCFAs formation in the gut. L. plantarum, as a probiotics, can be translated into a novel microbiota-targeted approach for managing metabolic and neurodegenerative diseases.