Details

Autor(en) / Beteiligte

• Liu, Edwin
• McDaniel, Kristen
• Case, Stephanie
• Yu, Liping
• Gerhartz, Bernd
• Ostermann, Nils
• Fankhauser, Gabriela
• Hungerford, Valerie
• Zou, Chao
• Luyten, Marcel
• Seidl, Katherine J.
• Michels, Aaron W.
• Nenna, Raffaella
• Raffaella Nenna

Titel

Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease

Ist Teil von

• Autoimmune Diseases, 2014-01, Vol.2014 (2014), p.927190-8

Ort / Verlag

United States: Hindawi Limiteds

Erscheinungsjahr

2014

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Class II major histocompatibility molecules confer disease risk in Celiac disease (CD) by presenting gliadin peptides to CD4 T cells in the small intestine. Deamidation of gliadin peptides by tissue transglutaminase creates immunogenic peptides presented by HLA-DQ2 and DQ8 molecules to activate proinflammatory CD4 T cells. Detecting gliadin specific T cell responses from the peripheral blood has been challenging due to low circulating frequencies and heterogeneity in response to gliadin epitopes. We investigated the peripheral T cell responses to alpha and gamma gliadin epitopes in young children with newly diagnosed and untreated CD. Using peptide/MHC recombinant protein constructs, we are able to robustly stimulate CD4 T cell clones previously derived from intestinal biopsies of CD patients. These recombinant proteins and a panel of α - and γ -gliadin peptides were used to assess T cell responses from the peripheral blood. Proliferation assays using peripheral blood mononuclear cells revealed more CD4 T cell responses to α -gliadin than γ -gliadin peptides with a single deamidated α -gliadin peptide able to identify 60% of CD children. We conclude that it is possible to detect T cell responses without a gluten challenge or in vitro stimulus other than antigen, when measuring proliferative responses.