Details

Autor(en) / Beteiligte

• Huang, Xiaolong
• Kong, Fandong
• Zhou, Shuangqing
• Huang, Dongyi
• Zheng, Jiping
• Zhu, Weiming

Titel

Streptomyces tirandamycinicus sp. nov., a Novel Marine Sponge-Derived Actinobacterium With Antibacterial Potential Against Streptococcus agalactiae

Ist Teil von

• Frontiers in microbiology, 2019-03, Vol.10, p.482-482

Ort / Verlag

Switzerland: Frontiers Media S.A

Erscheinungsjahr

2019

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• A novel actinobacterium, strain HNM0039 , was isolated from a marine sponge sample collected at the coast of Wenchang, Hainan, China and its polyphasic taxonomy was studied. The isolate had morphological and chemical characteristics consistent with the genus . Based on the 16S rRNA gene sequence analysis, strain HNM0039 was closely related to CGMCC 4.7042 (99.38%) and HNM0071 (99.05%). The organism formed a well-delineated subclade with CGMCC 4.7042 and HNM0071 in the 16S rRNA gene tree. Multi-locus sequence analysis (MLSA) based on five house-keeping gene alleles ( D, gyrB, rpoB, recA, trpB) further confirmed their relationship. DNA-DNA relatedness between strain HNM0039 and its closest type strains, namely CGMCC 4.7042 and HNM0071 , were 46.5 and 45.1%, respectively. The average nucleotide identity (ANI) between strain HNM0039 and its two neighbor strains were 89.65 and 91.44%, respectively. The complete genome size of strain HNM0039 was 7.2 Mbp, comprising 6226 predicted genes with DNA G+C content of 72.46 mol%. Thirty-one putative secondary metabolite biosynthetic gene clusters were also predicted in the genome of strain HNM0039 . Among them, the tirandamycin biosynthetic gene cluster has been characterized completely. The crude extract of strain HNM0039 exhibited potent antibacterial activity against in Nile tilapia. And tirandamycins A and B were further identified as the active components with MIC values of 2.52 and 2.55 μg/ml, respectively. Based on genotypic and phenotypic characteristics, it is concluded that strain HNM0039 represents a novel species of the genus whose name was proposed as sp. nov. The type strain is HNM0039 (= CCTCC AA 2018045 = KCTC 49236 ).