Details
- Autor(en) / Beteiligte
- Titel
- The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane
- Ist Teil von
- eLife, 2019-01, Vol.8
- Ort / Verlag
- England: eLife Sciences Publications Ltd
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is asymmetric and contains an outer leaflet of lipopolysaccharides (LPS) or lipooligosaccharides (LOS) and an inner leaflet of glycerophospholipids (GPL). We screened transposon mutants and identified a number of mutants with OM defects, including an ABC transporter system homologous to the Mla system in We further show that this opportunistic, antibiotic-resistant pathogen uses this multicomponent protein complex and ATP hydrolysis at the inner membrane to promote GPL export to the OM. The broad conservation of the Mla system in Gram-negative bacteria suggests the system may play a conserved role in OM biogenesis. The importance of the Mla system to OM integrity and antibiotic sensitivity suggests that its components may serve as new antimicrobial therapeutic targets.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/elife.40171Titel-ID: cdi_doaj_primary_oai_doaj_org_article_3e878368b67745b59962862f6cdc5c94
- Format
- –
- Schlagworte
- ABC Transporter, Acinetobacter baumannii, Acinetobacter baumannii - genetics, Acinetobacter baumannii - metabolism, Adenosine Triphosphate - chemistry, Anti-Bacterial Agents - pharmacology, Antibiotic resistance, Antibiotics, Bacteria, Bacterial Outer Membrane Proteins - metabolism, Biological Transport, Cell Membrane - metabolism, Computational Biology, Cryoelectron Microscopy, Defects, DNA Transposable Elements, E coli, Enzymes, Escherichia coli - metabolism, Escherichia coli Proteins - metabolism, Genes, Genome, Bacterial, Genotype & phenotype, glycerophospholipids, Glycerophospholipids - metabolism, Gram-negative bacteria, Hydrolysis, Lipids, Lipooligosaccharides, Lipopolysaccharides, Lipopolysaccharides - metabolism, Membrane permeability, Membranes, Microbiology and Infectious Disease, Molecular Conformation, Mutagenesis, Mutation, Nutrients, Opportunist infection, Permeability, Phenotype, Phosphatase, Proteins, Therapeutic applications