Details

Autor(en) / Beteiligte

• Iulietto, Maria Francesca
• Sechi, Paola
• Cella, Elisa
• Grispoldi, Luca
• Ceccarelli, Margherita
• Al Ani, Ahmad Rasheed
• Işıklar, Bilge
• Anil, Haluk M.
• Cenci-Goga, Beniamino Terzo

Titel

Inhibition of Listeria monocytogenes by a formulation of selected dairy starter cultures and probiotics in an in vitro model

Ist Teil von

• Italian journal of animal science, 2018-10, Vol.17 (4), p.845-850

Ort / Verlag

Taylor & Francis

Erscheinungsjahr

2018

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Three strains of lactic acid bacteria (LAB) and a commercial probiotic were selected to evaluate their in vitro activity towards Listeria monocytogenes. The strains Lactococcus lactis ssp. lactis, strain 340, L. lactis ssp. lactis, strain 16; Lactobacillus casei ssp. casei, strain 208 and Enterococcus faecium UBEF-41 were inoculated into skim milk and brain heart infusion broth (BHI) to get an initial Lactococcus: Lactobacillus: E. faecium UBEF-41 ratio of 2:1:1 and a concentration of approximately 7 log cfu mL −1 until challenge vs. pathogen. L. monocytogenes ATCC 7644 was also inoculated in same media to get approximately 4 log cfu mL −1 . Growth curves in skim milk and BHI at 4, 10 and 30 °C, respectively were studied for: (i) LAB formulation; (ii) L. monocytogenes and (iii) LAB vs. L. monocytogenes. When challenged with LAB, at 30 °C in milk, L. monocytogenes was not detectable after day-3 and in BHI it decreased below log cfu mL −1 after day-5. At 10 and 4 °C, in both media, L. monocytogenes counts were always significantly lower (p < .001) than the counts of L. monocytogenes alone from day-2 for milk at 4 °C and BHI at 10 °C and from day-7 for BHI at 4 °C and milk at 10 °C. In conclusion, the proposed formulation was able to limit L. monocytogenes in vitro growth, even at refrigeration temperature.