Clinical & developmental immunology, 2011-01, Vol.2011 (2011), p.1-12
2011
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- Autor(en) / Beteiligte
- Titel
- Development of Type 1 Diabetes Mellitus in Nonobese Diabetic Mice Follows Changes in Thymocyte and Peripheral T Lymphocyte Transcriptional Activity
- Ist Teil von
- Clinical & developmental immunology, 2011-01, Vol.2011 (2011), p.1-12
- Ort / Verlag
- Cairo, Egypt: Hindawi Puplishing Corporation
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- As early as one month of age, nonobese diabetic (NOD) mice feature pancreatic infiltration of autoreactive T lymphocytes, which destruct insulin-producing beta cells, producing autoimmune diabetes mellitus (T1D) within eight months. Thus, we hypothesized that during the development of T1D, the transcriptional modulation of immune reactivity genes may occur as thymocytes mature into peripheral T lymphocytes. The transcriptome of thymocytes and peripheral CD3+ T lymphocytes from prediabetic or diabetic mice analyzed through microarray hybridizations identified 2,771 differentially expressed genes. Hierarchical clustering grouped mice according to age/T1D onset and genes according to their transcription profiling. The transcriptional activity of thymocytes developing into peripheral T lymphocytes revealed sequential participation of genes involved with CD4+/CD8+ T-cell differentiation (Themis), tolerance induction by Tregs (Foxp3), and apoptosis (Fasl) soon after T-cell activation (IL4), while the emergence of T1D coincided with the expression of cytotoxicity (Crtam) and inflammatory response genes (Tlr) by peripheral T lymphocytes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1740-2522eISSN: 1740-2530DOI: 10.1155/2011/158735Titel-ID: cdi_doaj_primary_oai_doaj_org_article_3d70f68105dd49ce86781ea8b41241ec
- Format
- –
- Schlagworte
- Animals, Apoptosis, CD4-Positive T-Lymphocytes - immunology, CD4-Positive T-Lymphocytes - metabolism, CD8-Positive T-Lymphocytes - immunology, CD8-Positive T-Lymphocytes - metabolism, Diabetes Mellitus, Type 1 - genetics, Diabetes Mellitus, Type 1 - immunology, Diabetes Mellitus, Type 1 - metabolism, Diabetes Mellitus, Type 1 - physiopathology, fas Receptor - genetics, fas Receptor - metabolism, Female, Forkhead Transcription Factors - genetics, Forkhead Transcription Factors - metabolism, Gene Expression Profiling - methods, Genome-Wide Association Study - methods, Genomics - methods, Immunoglobulins - genetics, Immunoglobulins - metabolism, Interleukin-4 - pharmacology, Lymphocyte Activation - genetics, Mice, Mice, Inbred NOD, Oligonucleotide Array Sequence Analysis, Proteins - genetics, Proteins - metabolism, Thymus Gland - immunology, Thymus Gland - metabolism, Thymus Gland - pathology, Toll-Like Receptors - genetics, Toll-Like Receptors - metabolism, Transcription, Genetic