Details

Autor(en) / Beteiligte

• Sun, Kaitlyn A
• Li, Yan
• Meliton, Angelo Y
• Woods, Parker S
• Kimmig, Lucas M
• Cetin-Atalay, Rengül
• Hamanaka, Robert B
• Mutlu, Gökhan M

Titel

Endogenous itaconate is not required for particulate matter-induced NRF2 expression or inflammatory response

Ist Teil von

• eLife, 2020-04, Vol.9

Ort / Verlag

England: eLife Science Publications, Ltd

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Particulate matter (PM) air pollution causes cardiopulmonary mortality via macrophage-driven lung inflammation; however, the mechanisms are incompletely understood. RNA-sequencing demonstrated ( ) as one of the top genes induced by PM in macrophages. encodes a mitochondrial enzyme that produces itaconate, which has been shown to exert anti-inflammatory effects via NRF2 after LPS. Here, we demonstrate that PM induces Acod1 and itaconate, which reduced mitochondrial respiration via complex II inhibition. Using mice, we found that Acod1/endogenous itaconate does not affect PM-induced inflammation or NRF2 activation in macrophages in vitro or in vivo. In contrast, exogenous cell permeable itaconate, 4-octyl itaconate (OI) attenuated PM-induced inflammation in macrophages. OI was sufficient to activate NRF2 in macrophages; however, NRF2 was not required for the anti-inflammatory effects of OI. We conclude that the effects of itaconate production on inflammation are stimulus-dependent, and that there are important differences between endogenous and exogenously-applied itaconate.