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Comprehensive Bioinformatics Analysis Identifies POLR2I as a Key Gene in the Pathogenesis of Hypertensive Nephropathy
Ist Teil von
Frontiers in genetics, 2021-08, Vol.12, p.698570-698570
Ort / Verlag
Frontiers Media S.A
Erscheinungsjahr
2021
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
Hypertensive nephropathy (HN), mainly caused by chronic hypertension, is one of the major causes of end-stage renal disease. However, the pathogenesis of HN remains unclarified, and there is an urgent need for improved treatments. Gene expression profiles for HN and normal tissue were obtained from the Gene Expression Omnibus database. A total of 229 differentially co-expressed genes were identified by weighted gene co-expression network analysis and differential gene expression analysis. These genes were used to construct protein–protein interaction networks to search for hub genes. Following validation in an independent external dataset and in a clinical database,
POLR2I
, one of the hub genes, was identified as a key gene related to the pathogenesis of HN. The expression level of
POLR2I
is upregulated in HN, and the up-regulation of
POLR2I
is positively correlated with renal function in HN. Finally, we verified the protein levels of
POLR2I in vivo
to confirm the accuracy of our analysis. In conclusion, our study identified
POLR2I
as a key gene related to the pathogenesis of HN, providing new insights into the molecular mechanisms underlying HN.