Details

Autor(en) / Beteiligte

• Zhang, Lu
• Xue, Xu
• Yan, Jie
• Yan, Li-Ying
• Jin, Xiao-Hu
• Zhu, Xiao-Hui
• He, Zhi-Zhu
• Liu, Jing
• Li, Rong
• Qiao, Jie

Titel

Cryobiological Characteristics of L-proline in Mammalian Oocyte Cryopreservation

Ist Teil von

• Chinese medical journal, 2016-08, Vol.129 (16), p.1963-1968

Ort / Verlag

China: Wolters Kluwer - Medknow Publications

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Background： L-proline is a natural, nontoxic cryoprotectant that helps cells and tissues to tolerate freezing in a variety of plants and animals. The use of L-proline in mammalian oocyte cryopreservation is rare. In this study, we explored the cryobiological characteristics of L-proline and evaluated its protective effect in mouse oocyte cryopreservation. Methods： The freezing property of L-proline was detected by Raman spectroscopy and osmometer. Mature oocytes obtained from 8-week-old B6D2F 1 mice were vitrified in a solution consisting various concentration of L-proline with a reduced proportion ofdimethyl sulfoxide （DMSO） and ethylene glycol （EG）, comparing with the control group （15% DMSO and 15% EG without L-proline）. The survival rate, 5-methylcytosine （5-mC） expression, fertilization rate, two-cell rate, and blastocyst rate in vitro were assessed by immunofluorescence and in vitro fertilization. Data were analyzed by Chi-square test. Results： L-proline can penetrate the oocyte membrane within 1 min. The osmotic pressure of 2.00 mol/L L-proline mixture is similar to that of the control group. The survival rate of the postthawed oocyte in 2.00 mol/L L-proline combining 7.5% DMSO and 10% EG is significantly higher than that of the control group. There is no difference of 5-mC expression between the L-proline combination groups and control. The fertilization rate, two-cell rate, and blastocyst rate in vitro from oocyte vitrified in 2.00 mol/L L-proline combining 7.5% DMSO and 10% EG solution are similar to that of control. Conclusions： It indicated that an appropriate concentration of L-proline can improve the cryopreservation efficiency of mouse oocytes with low concentrations of DMSO and EG, which may be applicable to human oocyte vitrification.