Viruses, 2020-12, Vol.12 (12), p.1404
2020
Details
- Autor(en) / Beteiligte
- Titel
- HIV-1C env and gag Variation in the Cerebrospinal Fluid and Plasma of Patients with HIV-Associated Cryptococcal Meningitis in Botswana
- Ist Teil von
- Viruses, 2020-12, Vol.12 (12), p.1404
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- HIV-1 compartmentalization in reservoir sites remains a barrier to complete HIV eradication. It is unclear whether there is variation in HIV-1 and between cerebrospinal fluid (CSF) and plasma of individuals with HIV-associated cryptococcal meningitis (CM). We compared HIV-1 characteristics and the cytotoxic T-lymphocyte (CTL) escape mutations from CSF and plasma samples. Employing population-based Sanger sequencing, we sequenced HIV-1 from CSF of 25 patients and plasma of 26 patients. For , 15 CSF and 21 plasma samples were successfully sequenced. Of these, 18 and 9 were paired and CSF/plasma samples, respectively. There was no statistically significant difference in the proportion of CCR5-using strains in the CSF and plasma, ( = 0.50). Discordant CSF/plasma virus co-receptor use was found in 2/18 pairs (11.1%). The polymorphisms in the HIV-1 V3 loop were concordant between the two compartments. From the HIV-1 sequences, three pairs had discordant CTL escape mutations in three different epitopes of the nine analyzed. These findings suggest little variation in the HIV-1 between plasma and CSF and that the CCR5-using strains predominate in both compartments. HIV-1 CTL escape mutations also displayed little variation in CSF and plasma suggesting similar CTL selective pressure.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1999-4915eISSN: 1999-4915DOI: 10.3390/v12121404Titel-ID: cdi_doaj_primary_oai_doaj_org_article_3c0f3b66668a4526ac4d9fc9da4430b7
- Format
- –
- Schlagworte
- Adult, AIDS-Related Opportunistic Infections - blood, AIDS-Related Opportunistic Infections - cerebrospinal fluid, AIDS-Related Opportunistic Infections - diagnosis, AIDS-Related Opportunistic Infections - metabolism, Amino Acid Sequence, Amino Acid Substitution, Botswana, Brain research, CCR5, CCR5 protein, CD4 Lymphocyte Count, Cerebrospinal fluid, co-receptor, Cross-Sectional Studies, cryptococcal meningitis, CXCR4, Cytotoxicity, Deoxyribonucleic acid, Disease Susceptibility, DNA, env Gene Products, Human Immunodeficiency Virus - blood, env Gene Products, Human Immunodeficiency Virus - cerebrospinal fluid, env Gene Products, Human Immunodeficiency Virus - metabolism, Epitopes, Female, gag Gene Products, Human Immunodeficiency Virus - blood, gag Gene Products, Human Immunodeficiency Virus - cerebrospinal fluid, gag Gene Products, Human Immunodeficiency Virus - metabolism, Gag protein, Glycoproteins, HIV, HIV Infections - complications, HIV Infections - virology, Human immunodeficiency virus, Humans, Immunocompromised Host, Infections, Lymphocytes T, Male, Meningitis, Meningitis, Cryptococcal - blood, Meningitis, Cryptococcal - cerebrospinal fluid, Meningitis, Cryptococcal - etiology, Meningitis, Cryptococcal - metabolism, Middle Aged, Mutation, Patients, Plasma, RNA, Viral, Statistical analysis, Strains (organisms), Variation, Viral Load