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Dominant Elongase Activity of Elovl5a but Higher Expression of Elovl5b in Common Carp ( Cyprinus carpio )
Ist Teil von
International journal of molecular sciences, 2022-11, Vol.23 (23), p.14666
Ort / Verlag
Switzerland: MDPI AG
Erscheinungsjahr
2022
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Most diploid freshwater and marine fish encode one
elongase, having substrate specificity and activities towards C18, C20 and C22 polyunsaturated fatty acids (PUFAs). The allo-tetraploid common carp is hypothesized to encode two duplicated
genes. How these two
genes adapt to coordinate the PUFA biosynthesis through elongase function and expression divergence requires elucidation. In this study, we obtained the full-length cDNA sequences of two
genes in common carp, named as
and
. Functional characterization showed that both enzymes had elongase activity towards C18, C20 and C22 PUFAs. Especially, the activities of these two enzymes towards C22 PUFAs ranged from 3.87% to 8.24%, higher than those in most freshwater and marine fish. The Elovl5a had higher elongase activities than Elovl5b towards seven substrates. The spatial-temporal expression showed that both genes co-transcribed in all tissues and development stages. However, the expression levels of
were significantly higher than those of
in all examined conditions, suggesting that
would be the dominantly expressed gene. These two genes had different potential transcriptional binding sites. These results revealed the complicated roles of
on PUFA synthesis in common carp. The data also increased the knowledge of co-ordination between two homoeologs of the polyploid fish through function and expression divergence.